ÄKTA Pure 25 Chromatography System
A versatile, research-grade FPLC for protein purification at lab scale — modular, UNICORN-controlled, and easy to reconfigure between methods.
Independent reseller — not affiliated with Cytiva. Sold with our own functional guarantee. See disclaimer.
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Overview
The ÄKTA Pure 25 is one of the most widely used FPLC systems in protein science, built around interchangeable modules for affinity, ion-exchange, size-exclusion and multi-step purifications. It runs under UNICORN software with flow rates suited to bench-scale work and supports a broad range of columns and detectors.
We supply the ÄKTA Pure 25 refurbished and function-tested — pumps primed and holding pressure, flow path checked, and the exact module and detector configuration confirmed in writing. Tell us your target method and we'll match a system, software and PC to it.
Specifications
| Flow rate | 0.001–25 mL/min |
|---|---|
| Max pressure | 20 MPa |
| UV detection | Single or multi-wavelength |
| Software | UNICORN |
Specifications reflect the manufacturer's standard configuration. Exact specs for the unit you're quoted are confirmed in writing.
Typical use cases
- Protein and antibody purification
- Downstream process development
- Method scouting across modes
- Pilot-scale biologics work
Condition
Refurbished — inspected & function-tested. Full condition notes and any service history are shared before purchase.
Frequently asked questions
What are the key specifications of the ÄKTA Pure 25 Chromatography System?
As standard it offers flow rate 0.001–25 mL/min, max pressure 20 MPa, uv detection Single or multi-wavelength. We confirm the exact configuration of the individual unit in your quote.
What condition is it in, and do you offer support?
This is a quality-verified pre-owned Cytiva unit, inspected and function-tested before shipment. We share condition notes, confirm software/accessory status, and pack it professionally for worldwide freight.